Pour The combination of lysate and Ni‐NTA agarose to the column, and allow it to empty both by gravity move or by implementing a vacuum to The underside of the column. A supplementary protocol allows optional purification of protein. The purified protein is denatured and may be used in purposes https://www.facebook.com/NewTechFind/posts/pfbid028suFpZc3zrsMyMH2s35rRvKiiV5ZaLFRtKXBWSmig5vMs847oSeFLEF184pRjxtfl